絲氨酸/蘇氨酸激酶AKT多克隆抗體

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產(chǎn)品英文名稱	Akt Polyclonal Antibody
免疫原	合成多肽：人源蛋白Akt，非磷酸化多肽，中心氨基酸為T308.
宿主	兔
反應(yīng)性	人, 大鼠, 小鼠
應(yīng)用	ELISA, IHC-P, WB
實(shí)驗(yàn)建議	最佳的工作稀釋比例需要客戶摸索優(yōu)化。建議的起始嘗試的稀釋比為：WB：1:500-1:2000，IHC-p：1:100-1:300，ELISA：1:20000。尚未在其他應(yīng)用中測(cè)試。
克隆性	多克隆
純化工藝	使用表位特異性的免疫原，通過(guò)親和層析，從兔抗血清中親和純化抗體。

產(chǎn)品形式	液體溶液
濃度	1 mg/ml
儲(chǔ)存緩沖液	含有50％甘油，0.5％BSA和0.02％疊氮化鈉的PBS緩沖液。
保存建議	自發(fā)貨之日起，-20°C可穩(wěn)定保存1年。為最大限度的避免損失，請(qǐng)?jiān)诖蜷_管蓋之前融化抗體并離心。我們建議使用前分裝以避免反復(fù)凍融。
運(yùn)輸條件	藍(lán)冰運(yùn)輸
警告	本文列出的產(chǎn)品僅供研究使用，不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何專利或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的專利侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。

背景	The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1.
基因ID	207/208/10000
別名	AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B
其它	該抗體可檢測(cè)內(nèi)源蛋白
蛋白質(zhì)ID	P31749/P31751/Q9Y243